Results Apoptotic cells mainly appeared in the inner nuclear and the ganglion cell layers, almost no positive expression was seen in the outer nuclear layer. 结果凋亡细胞主要出现在视网膜内核层及神经节细胞层,而外核层几乎无阳性表达。
Morphological examination: The retinal thickness of outer nuclear layer and photoreceptor cells in control group was ( 98.4 ± 1.8) μ m. 2)形态学检查测到正常组大鼠中心视网膜的外视网膜厚度为(98.4±1.8)μm。
By electron microscope, the apoptosis of the outer nuclear layer cells were increased from the 14th day; 电镜结果显示,rds小鼠视网膜的外核层细胞从出生后第14天凋亡细胞增加;
Results The positive staining of Fos protein was found in ganglion cell layer, inner nuclear layer and pigment epithelium, but not in outer nuclear layer 30 min after perfusion. 结果灌注术后30min,在节细胞层、内颗粒层及色素上皮层出现Fos蛋白阳性染色。
The apoptosis of the outer nuclear layer cell apoptosis was detected by TUNEL technigue and the immunohistochemistry method was used to detect Bcl-2 expression in the retina. 用TUNEL方法检测视网膜光感受器细胞的凋亡,用免疫组化方法测定Bcl-2在视网膜的表达。
On the 1st day after light-exposure, the outer nuclear layer became thinner by 11 88%, nuclear chromatin began to shrink. 光照后1天,外核层开始变薄,其厚度减少1188%。
The apoptosis had lower expression in ganglion ceils and the inner and outer nuclear layer 6 hours latter, and obvious increasing of dye positive eeUs was observed between 12 and 24 hours. 细胞凋亡6h在神经节细胞和内、外核层有少量表达,12~24h染色阳性的细胞明显增多。
Compared with young cats, the retinae in old cats presented a significant decrease in the thickness of total retina, outer nuclear layer, outer plexiform layer, inner nuclear layer, inner plexiform layer as well as an evidently lower density of ganglion cells. 与青年猫比较,老年猫视网膜总厚度以及外核层、外网状层、内核层和内网状层厚度均显著减小;神经节细胞层的细胞密度显著下降;
The number of nucleus layers, in the order of outer nuclear layer, inner nuclear layer and ganglion cell layer are 2. 6, 7. 6 and 0. 4 in B. bubo; 视网膜外核层、内核层和节细胞层胞核的平均层数在依次为2.6、76和0.4层;
On postnatal 7th day, VEGF expressed in ganglion cell layer, outer plexiform layer and almost all the inner nuclear layer. 7d时,VEGF明显表达在神经节细胞层、外丛状层和几乎全层内核层。
In four-month diabetic group, VEGF expression in choroid was positive ( 66.7%), and VEGF expression in inner limiting membrane, inner nuclear layer, outer nuclear layer and ganglion cell layer of retina was positive ( 77.8%). 糖尿病4个月组(M4)脉络膜VEGF蛋白表达阳性(66.7%),视网膜的内界膜、内核层、外核层及节细胞层VEGF蛋白表达阳性(77.8%)。
Apoptosis cells were observed in detached retina, mainly in inner nuclear layer, outer nuclear layer and ganglionic cell layer. 脱离的视网膜在内、外核层发现凋亡标记阳性细胞。
The nuclear chromatin in the outer nuclear layer migrated toward the center with an uneven distribution. 外核层细胞核染色质固缩,分布不均匀,向中央聚集。
The outer nuclear layer and photoreceptor layer were almost completely lost at 7 d. 7d后,外颗粒层和光感受层几乎完全消失。
Conclusions Puerarin could postpone lesion occurrence of retinal outer nuclear layer cell and photoreceptor outer segment. 结论葛根素可延缓rd小鼠视网膜外核层细胞和视网膜光感受器外段损伤。
After 36 hour light exposure, the rod outer and inner segments were lysed and most of the nuclei in the outer nuclear layer were disappeared. 36小时后,视杆细胞内、外节溶解,外核层大量细胞核丢失。
EGF immunostaining was not strong, but it was specific and distributed uniformly at the ganglion cell layer ( GCL), inner nuclear layer ( INL), outer nuclear layer ( ONL), and retinal pigment epithelium layer ( RPE); EGF免疫反应着色不强,但有特异性地均匀分布在节细胞层(GCL)、内核层(INL)、外核层(ONL)和视网膜色素上皮层(RPE);
With ⅱ grade photic spot, many RPE cells, photoreceptors and cells in outer nuclear layer were lysed and destroyed; 级光斑示rpe、感光细胞和外核层细胞大量溶解破坏;
Results All paris of the photoreceptor cell in the sheet maintained intact, including the outer segments, inner segments, outer limiting membrane, outer nuclear layer, and outer plexiform layer. 结果切削后所得视细胞植片由单层视细胞组成,结构完整,包括外丛状层、外核层和外节层;
The expression of VIP exists on photoreceptor-outer segment, inner nuclear layer and inner plexiform layer of retina and choroids, and weak expression exists on ganglion cell and nerve fiber layer. VIP表达主要位于视网膜光感受器外节、内核层、内网状层和脉络膜,神经节细胞层和神经纤维层也有弱表达;
Pathological change of retina: retina structure is each level clearly demarcated in Group S, the outer nuclear layer arrangement is close, the inside and outside arrange in neat rows rule, and the dividing line is clear. 视网膜组织病理变化:S组视网膜结构层次分明,外核层排列紧密,内外节排列整齐规则,分界清晰。
HE staining, transmission electron microscopy and TUNEL were used to detect photoreceptor apoptosis process in outer nuclear layer. 采用HE染色,透射电镜和TUNEL染色方法检测视网膜外核层感光细胞的凋亡过程。